Identification of Some Streptomycetes Producing Oxytetracycline

Transduction of plasmid DNA in macrolide producing streptomycetes.

Transduction was mediated by a 8-kb segment of bacteriophage FP43 DNAcloned into plasmid pIJ702: The resulting plasmid, pRHBlOl, was packaged in bacteriophage heads as linear concatemers and was transduced into about 80% of the Streptomyces species tested1}. We showed that the efficiency of transduction can vary markedly depending on the cell growth conditions, and that under optimum growth con...

Molecular detection of streptomycin-producing streptomycetes in Brazilian soils.

Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultiv...

Growth Phases of Some Antibiotics Producing Streptomyces and their Identification

The growth phases of five cultures of antibiotic producing Streptomyces isolated from western region, Saudi Arabia were studied spe. cial reference to their potentiality to excrete antibiotics. It was found that these organisms grew exponentially during the first 4 to 7 days of incubation. The highest specific growth "rate was observed in the case of Streptomyces MY18 being 0.48 day-l (1.44 day...

rapid isolation and molecular detection of streptomycin-producing streptomycetes

introduction: streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. the gene cluster for the production of streptomycin antibiotic contains strr gene that encodes strr, a pathway-specific regulator. then, this p...

Sporulation by some strains of nocardiae and streptomycetes.